Tiger Roll won the race which saw half of the field fail to finish the famous steeplechase race
Up For Review suffered a fatal injury after falling at the first fence of the Grand National.
The horse, a 25-1 shot, was put down after the race which saw Tiger Roll win for a second successive year at Aintree.
He is the first Grand National horse to be put down since 2012.
General Principle was taken away in a horse ambulance, a day after two horses were put down on the penultimate day of the festival.
The hot favourite, ridden by jockey Davy Russell, kept out of trouble in the 40-horse field to emulate the feat of National great Red Rum 45 years ago.
Tiger Roll, the smallest horse in the race, won by three lengths from Magic of Light, Rathvinden and Walk In The Mill.
Winning owner Michael O’Leary said: “It’s unbelievable. It’s a phenomenal training performance by Gordon. It’s brilliant that he keeps bringing this horse back at Cheltenham better than ever and Aintree better than ever
And what a ride by Davy – fantastic. It’s unbelievable, to win two Grand Nationals is just incredible.
“It’s a great result for the punters as well.”
For much of the way it looked like Ruby Walsh might seal his third National success as his mount Rathvinden raced and jumped with zest on the front end, along with stablemate and last year’s narrowly beaten runner-up Pleasant Company.
However, Tiger Roll was always in their slipstream and it was clear on the run to the final fence that barring accidents he would seal victory, with Russell still motionless in the saddle.
Jessica Harrington’s mare Magic Of Light attempted to chase him down, but Tiger Roll was not for catching and passed the post comfortably ahead.
It was a third National success for Elliott – having first struck gold as a fledgling trainer with Silver Birch in 2007.